Thymus-Derived ILC2s From Id1tg/tg Mice Protect Against aGVHD

Srinivasan A, Bajana S, Pankow A, et al. Type 2 Innate Lymphoid Cells From Id1 Transgenic Mice Alleviate Skin Manifestations of Graft-Versus-Host Disease. BMC Immunology. 2021; (doi: 10.1186/s12865-021-00432-w).

Thymus-derived activated Group 2 innate lymphoid cells (ILC2s) from genetically engineered mice that express Id1 protein (Id1tg/tg) protect against acute graft-versus-host disease (aGVHD), according to new research. Wild-type (WT) ILC2s have a protective effect against aGVHD, and the researchers sought to determine whether thymus-derived ILC2s offer such protection as well. For the study, a bone marrow transplant was performed by irradiating a BALB/c strain of recipient mice and transplanting with bone marrow and T cells from the MHC-disparate C57BL/6 strain. ILC2s from Id1tg/tg mice and ones with E protein genes knocked out (dKO) in the thymus were isolated and then co-transplanted to evaluate their effects. Activated ILC2s were confirmed to have a protective role against aGVHD; however, the origin of the ILC2s affected how they worked. ILC2s from dKO mice were not as effective in protecting against aGVHD and reacted differently depending on it the cells isolated were pre-treated with IL-25 in vivo. ILC2s co-transplanted from Id1tg/tg mice were protective against aGVHD, also offering significant improvement against skin lesions from aGVHD.

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